Isolation of Antibiotic Compound Producing Bacteria from the Soil Rhizosphere Against Escherichia Coli and Streptococcus Aureus
Keywords:
Rhizosphere soil, Rhizosphere bacteria, Antibiotic compoundAbstract
This research aims to isolate bacteria from the rhizosphere soil which are known to be good media for the growth of bacteria, such as Escherichia coli and Straphlococus aureus as new types of antibiotics. The method used by isolated the bacteria from rhizosphere soil of Rimpang Pacing and Guava rhizome plants and then do the cell morphology characterization, purification, propagation of antibiotic-producing bactrial isolates and continued testing the antibiotic activity of potential bacterial isolates. From the observations, it was found that the isolation of bacteria from the rhizosphere soil of the pacing rhizome had antibiotic compounds that were able to inhibit the growth of Escherichia coli and Straphlococcus aureus bacteria as indicated by the formation of a clear zone around the colony, which had an inhibitory index of 0.5 mm in Ec.2 and Bp.1 isolates is also 0.5 mm. From Both Ec.2 and Bp.1 isolates concluded that the rhizosphere soil produces an inhibitory zone that has the potential to be used as an antibiotic agent
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Angraini E, Angraeni D. N, Umami S. S, Sumiati E, dan Taufiqurokhman T. 2019. Antagonism of Lentinus cladopus Lc4 Extract, Trichoderma sp. Jpa Extract on Bacillus sp., Xanthomonas sp. and E. coli. In Journal of Physics: Conference Series. 1155 (1) p. 012057). IOP Publishing.
Arora S, Nandi D, Prasad N, Rawat S, Pandey A, University GE, Dehradun, Science ML. 2013. Isolation and characterization of antibiotic producing microbes present in rhizospheric soil. Internat J Sci Engineer Res. 4(9): 1157-1166.
Ferfinia A. 2010. Eksplorasi bakteri dan cendawan rizofer yang berasosiasi dengan penyakit busuk basah pada batang pepaya (Carica papaya) di Pasir Kuda, Desa Ciomas, Bogor. Skripsi. Departemen Pertanian Fakultas Pertanian IPB. Bogor. Diakses melalui http://repository.ipb.ac.id/handle/123456789/26878. Pada tanggal 20 Maret 2019.
Cetina A, Matos A, Garma G, Barba H, Vazquez R, Rodriguez AZ, Jay D, Monteon V, Lopez R. 2010. Antimicrobial activity of marine bacteria isolated from Gulf of Mexico. Rev Peru Biol. 17(2): 231-236.
Hardianti F dan Aziz I. R. 2019. Identification of Pathogenic Bacteria on the Salted Fish Lutjanus Vivanus in Sorong City of West Papua. Malaysian J. of Microbiology. 15 (3): 237-244.
Martha D. 2015. Perbandingan Hasil Uji Kepekaan Escherichia coli terhadap Seftriakson dengan Siprofloksasin Di Rumah Sakit Al-Islam pada Tahun 2014.
Melander RJ and Melander C. 2017. The challenge of overcoming antibiotic resistence: an adjuvant approach. Americ Chem Soc. 12(2017): 1-5.
Mukamto, Syazwani U, Weda M, Ahmad S, Laila I, Guntur T. 2015. Isolasi dan karakterisasi Bacillus sp. pelarut fosfat dari rizosfer tanaman Leguminosae. J. Sains dan Teknologi. 3(2): 62-68.
Sethi S, Kumar R, Gupta S. 2013. Antibiotic production by microbes isolated from soil. Internat J Pharm Sci Res. 4(8): 2967-2973.
Singer RS, Finch R, Wegener HC, Bywater R, Walters J, Lipsitch M. 2003. Antibiotic resistance – the interplay between antibiotic use in animals and human beings. The Lancer infectious diasese. 3(1): 47-51.
Suarez C dan Gudiol, F. 2009. Betalactam antibiotics. Enfermedades infecciosas y microbiologia clinica. 27(2), 116-129.
Sukmawati S dan Hardianti F. 2018. Analisis Total Plate Count (TPC) Mikroba pada Ikan Asin Kakap di Kota Sorong Papua Barat. J. Biodjati. 3 (1): 72-78.
Sukmawati S, Ratna R dan Fahrizal A. 2018. Analisis Cemaran Mikroba pada Daging Ayam Broiler Di Kota Makassar. Scripta Biologica. 5 (1): 51-53.
Sulistiyani TR dan Nunuk W. 2011. Isolasi, seleksi dan identifikasi molekuler aktinomisetes penghasil antibiotik. J Widyariset. 14(3): 541-548.
Wahjudi M, Lutfia LA, Ruth C. 2014. Isolasi bakteri dari tanah Gunung Kapur dan pengujian aktivitas antibakteri isolat terhadap bakteri Escherichia coli dan Staphylococcus aureus. J. Sains dan Teknologi. 7(2): 7-17.
D. Ayu and A. Muchtar, “Overview Of The Condition Of Sanitation Facilities Where Food Management At Sultan Hasanuddin International Airport Makassar,†vol. 11, no. 01, pp. 1–8, 2020.
N. Natalia, “The Relationship Of Social Role On Depression In The Elderly In Candimulyo Village , Jombang District,†vol. 10, no. 02, pp. 1–7, 2020.
